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Por favor, use este identificador para citar o enlazar este ítem: https://hdl.handle.net/20.500.12104/72689
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Campo DCValorLengua/Idioma
dc.creatorCortés Garcia, Juan Diego-
dc.creatorLópez López, Cintya-
dc.creatorCortez Espinosa, Nancy-
dc.creatorGarcía Hernández, Mariana H.-
dc.creatorGuzmán Flores, Juan Manuel-
dc.creatorLayseca Espinosa, Esther-
dc.creatorPortales Cervantesa, Liliana-
dc.creatorPortales Pérez, Diana Patricia-
dc.date2016-09-28T18:39:57Z-
dc.date2016-09-28T18:39:57Z-
dc.date2016-01-
dc.date.accessioned2019-04-07T23:49:09Z-
dc.date.available2019-04-07T23:49:09Z-
dc.identifierCortés-Garcia JD, López-López C, Cortez-Espinosa N, García-Hernández MH, Guzmán-Flores JM, Layseca-Espinosa E., Portales-Cervantes L, Portales-Pérez DP. Evaluation of the expression and function of the P2X7 receptor and ART1 in human regulatory T-cell subsets. Immunobiology. 2016 Jan;221(1):84-93. doi: 10.1016/j.imbio.2015.07.018. Epub 2015 Jul 29.-
dc.identifier0171-2985-
dc.identifierdx.doi.org/10.1016/j.imbio.2015.07.018-
dc.identifierhttp://repositorio.cualtos.udg.mx:8080/jspui/handle/123456789/559-
dc.identifier.urihttps://hdl.handle.net/20.500.12104/72689-
dc.descriptionRegulatory T cells that express CD39 (CD39+ Treg) exhibit specific immunomodulatory properties. Ectonucleotidase CD39 hydrolyses ATP and ADP. ATP is a ligand of the P2X7 receptor and induces the shedding of CD62L and apoptosis. However, the role of ATP in CD39+ Treg cells has not been defined. Furthermore, NAD can activate the P2X7 receptor via ADP-ribosyltransferase (ART) enzymes and cause cell depletion in murine models. We evaluated the expression and function of P2X7 and ART1 in CD39+ Treg and CD39- Treg cells in the presence or absence of ATP and NAD. We isolated peripheral blood mononuclear cells from healthy subjects and purified CD4+ T cells, CD4+ CD25+ T cells and CD4+ CD25+ CD39+ T cells. P2X7 and ART1 expression was assessed by flow cytometry and real-time PCR. Our results showed low P2X7 expression on CD39+ Treg cells and higher levels of ART1 expression in CD4+ CD39+ T cells than the other subtypes studied. Neither shedding of CD62L nor cell death of CD39+ Treg or CD39- Treg cells was observed by 1mM ATP or 60μM NAD. In contrast, P2Xs receptor-dependent proliferation with 300μM ATP, was inhibited by NAD in the different cell types analysed. The NAD proliferation-inhibition was increased with P2Xs and A2a agonist and was reversed with P2Xs and A2a antagonist, therefore NAD inhibits P2Xs-dependent proliferation and A2a activation. In conclusion, our results suggest that the altered function and expression of P2X7 and ART1 in the human CD39+ Treg or CD39- Treg cells could participate in the resistance against cell death induced by ATP or NAD.-
dc.descriptionLaboratory of Immunology and Cellular and Molecular Biology, Faculty of Chemical Sciences, UASLP, San Luis Potosí, S.L.P., Mexico Division of Molecular Biology, Instituto Potosino de Investigación Científica y Tecnológica, San Luis Potosí, S.L.P. Mexico Unit of Medicine Investigation IMSS, Zacatecas, Zac, Mexico Department of Immunology, Faculty of Medicine, UASLP, San Luis Potosí, S.L.P. Mexico-
dc.languageen-
dc.publisherElsevier GmbH-
dc.relationImmunobiology.;Vol. 221. Número1. Pags. 84-93-
dc.subjectP2X7 receptor-
dc.subjectART1-
dc.subjectATP-
dc.subjectNAD-
dc.subjectimmune regulation-
dc.subjectCD39-
dc.titleEvaluation of the expression and function of the P2X7 receptor and ART1 in human regulatory T-cell subsets-
dc.typeArticle-
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